Journal: Cellular and Molecular Bioengineering

Article Title: Remote-Controlled Gene Delivery in Coaxial 3D-Bioprinted Constructs using Ultrasound-Responsive Bioinks

doi: 10.1007/s12195-024-00818-x

Figure Lengend Snippet: Effect of varying the number of ultrasound pulses on ultrasound-controlled osteoblast cellular transfection in coaxially-bioprinted constructs. A Coaxially-bioprinted osteoblast-laden (hFOB 1.19) 4% alginate bioink containing GFP-coupled microbubbles before ultrasound, 0 hr post-ultrasound, and 48 hr post-ultrasound with varying ultrasound exposure (10, 40, or 80 pulses). B Number of transfected cells in bioprinted constructs at 48 hr post-ultrasound for varying numbers of ultrasound pulses (*p < 0.05, ****p < 0.0001, one-way ANOVA, Tukey’s multiple comparisons test, error bars denote standard deviation). C Diameter of zone containing transfected cells at 48 hr post-ultrasound for varying numbers of ultrasound pulses (one-way ANOVA, Tukey’s multiple comparisons test, error bars denote standard deviation)

Article Snippet: They were then suspended in complete media containing Human Osteoblast Media (Cell Applications, Inc.), 10% FBS (HyClone Characterized Fetal Bovine Serum CA Origin, Cytiva), and 1% pen-strep (penicillin-streptomycin, 10,000 μg/mL, Gibco) then concentrated to 3.85 × 10 7 cell/mL in complete media.

Techniques: Transfection, Construct, Standard Deviation

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Quantitative Age-specific Variability of Plasma Proteins in Healthy Neonates, Children and Adults *

doi: 10.1074/mcp.M116.066720

Figure Lengend Snippet: ELISA validation of differentially expressed proteins. A, GPI-specific phospholipase 1 (GPLD1), B, Periostin (POSTN), C, Alpha-2-macroglobulin (A2M), D, Histidine rich glycoprotein (HRG). E, For comparison, boxplots showing the respective abundance patterns detected from the SWATH data using the extended library (GPLD1 and POSTN) and the local library (A2M and HRG). All error bars are standard deviation (SD). * Denotes significance ≤ 0.05; ** denotes significance ≤ 0.01.

Article Snippet: Enzyme-Linked Immunosorbent Assay (ELISA) validation We utilized the following ELISA kits according to the manufacturer's specifications: GPI-specific phospholipase D (GPLD1); (CSB-EL009721HU, Cusabio, Wuhan, China), Periostin (POSTN) (CSB-E16444H, Cusabio, Wuhan, China), Alpha-2-macroglobulin (A2M) (ab108888, Abcam, Cambridge, United Kingdom), Histidine rich glycoprotein (HRG) (CSB-E13159H, Cusabio, Wuhan, China).

Techniques: Enzyme-linked Immunosorbent Assay, Biomarker Discovery, Comparison, Data-independent acquisition, Standard Deviation

Journal: Skeletal Muscle

Article Title: Angiotensin II type 1 receptor antagonists alleviate muscle pathology in the mouse model for laminin-α2-deficient congenital muscular dystrophy (MDC1A)

doi: 10.1186/2044-5040-2-18

Figure Lengend Snippet: L-158809 lowered angiotensin II receptor type 1 (AT1) -mediated transforming growth factor (TGF)-β levels in the muscles of dy W /dy W mice. (A) AT1-mediated TGF-β levels were increased in muscles of dy W /dy W mice and decreased after L-158809 administration. Triceps cross-sections of dy W /dy W mice showed expression of TGF-β (green) and periostin (green), and nuclear accumulation of pSmad2/3 (green), which is a downstream target of AT1. Oral L-158809 treatment of dy W /dy W mice ( dy W -L158) reduced expression of all three proteins. Nuclei were visualized with 4",6"-diamidino-2-phenylindole hydrochloride (DAPI) (blue). (B) Western blotting analysis of triceps muscles confirmed increased protein expression levels of periostin (75 to 85 kDa) and TGF-β (12 kDa, reduced) in dy W /dy W mice as well as the reduction after L-158809 treatment. β-actin was used as a loading control. (C) The number of pSmad2/3-positive nuclei was more than 25-fold increased in cross-sections of dy W /dy W muscle compared with wild-type (WT) muscles, and was significantly reduced by L-158809 to levels 2-fold (triceps) and 2.6-fold (diaphragm) those of WT mice, which was not significant (n ≥ 4). (D) Quantitative real-time PCR showed an increase of approximately 3.5-fold in TGF-β1 mRNA levels in both dy W /dy W triceps and diaphragm muscles, which was reduced to nearly WT levels by L-158809 (n ≥ 4). (E) Thrombospondin-1 (green) was increased in both triceps and diaphragm muscles of dy W /dy W mice, and was minimized by L-158809 administration. All values represent the mean ± SEM. One-way ANOVA: ** P ≤ 0.001; * P ≤ 0.05; n.s. (non-significant) P > 0.05. Scale bar = 50 μm.

Article Snippet: Monoclonal rabbit anti-mouse TGF-β (56E4, CST #3709), monoclonal rabbit anti-human phospho-Smad2(Ser465/467)/Smad3(Ser423/425) antibody (CST #9510) (both Cell Signaling Technology, Beverly, MA, USA) polyclonal rabbit anti-human TSP 1 (LS-C26356; Lifespan Biosciences, Inc., Seattle, WA, USA), polyclonal rabbit anti-human periostin (RD181045050; BioVendor LLC, Candler, NC, USA), monoclonal rat anti-mouse F4/80 (ab6640; Abcam, Cambridge, MA, USA), monoclonal mouse anti-rat developmental myosin heavy chain (dMyHC) (NCL-MHCd; Novocastra, Norwell, MA, USA), monoclonal rat anti-mouse laminin-γ1 chain (MAB1914; Chemicon (now EMD Millipore, Billerica, MA, USA)).

Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction